Proton and Deuteron Relaxation Study of Molecular Dynamics in Lysozyme Solutions |
| J.F. Kakulea, W.P. Węglarzb, R.K. Shenoyc, A.R. Sharpa and H. Peemoellerc
a Department of Physics, University of New Brunswick, Fredericton New Brunswick, E3B 5A3, Canada, b H. Niewodniczański Institute of Nuclear Physics, Kraków, Poland, c Department of Physics, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada Received: August 23, 1999; revised version May 15, 2000 |
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| A nuclear magnetic resonance spin-lattice relaxation dispersion study of the relaxation of several magnetization components in both natural and deuterated lysozyme solutions was undertaken at 20°C. Proton and deuteron resonances were employed. The two-dimensional time evolution of the magnetization and the spin-spin relaxation were analyzed. In addition, an isotopic dilution study was performed at 5 and 30.6MHz. The results indicate that the water proton spin-lattice relaxation rate which arises from intermolecular relaxation between the water protons and the lysozyme protons represents a relatively strong relaxation mechanism. A model for the dynamics of the water molecules, consistent with the proton and deuteron dispersions as well as with the isotopic dilution results, is presented. |
| DOI: 10.12693/APhysPolA.98.131 PACS numbers: 87.15.-v, 76.60.-k, |