Label-Free Localized Surface Plasmon Resonance Biosensor Used to Detect Serum Interleukin-10 in Patients with Endometrial Cancer |
| L. Lia, b, C. Chenga, H. Yangc, H. Yea, X. Luoc, M. Xia, b
aGynecology Department of West China Second University Hospital, Sichuan University, Chengdu, 610041, China bKey Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, 610041, China cKey Laboratory of Optical Technologies for Microfabrication, Institute of Optics and Electronics, Chinese Academy of Science, Chengdu, 610029, China |
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| Our aim was to fabricate a localized surface plasmon resonance (LSPR) silver nanostructure immune biosensor, and evaluate its efficiency in detecting serum interleukin-10 (IL-10) cytokine in endometrial cancer patients. A triangle silver nanostructure was designed with the use of the finite-difference time-domain (FDTD) numerical method, fabricated by nanosphere lithography (NSL), modified by an 11-mercaptoundecanoic acid (MUA) and anti-human interleukin-10 monoclonal antibody. The fabricated LSPR sensor chip was taken to detect a gradient concentration of recombinant IL-10 protein, and characterized by a scanning electron microscope (SEM), and an atomic force microscope (AFM). Finally, the serum of endometrial cancer (n1=9) and benign uterine disease (n2=9) were respectively detected by the LSPR sensor and enzyme-linked immune-adsorbent assay (ELISA) kit. The LSPR biosensor could detect commercial IL-10 or IL-10 in serum samples within 30 min and the spectrum signal (Δλmax)of serum IL-10 in the endometrial cancer group was higher than the signal of the benign group (15.30±6.22 nm vs 8.13±2.57 nm, p=0.009), which was consistent with that of ELISA (405.2±56.1 pg/ml vs. 162.2±57.4 pg/ml, p=0.017). The LSPR triangle silver nanostructure biosensor provides a promising platform with attractive advantages for a serological diagnosis in endometrial cancer, but further optimization of this biosensor is necessary for its transfer to clinical application. |
DOI:10.12693/APhysPolA.138.338 topics: localized surface plasmon resonance, metal nanostructure, biosensor, interleukin-10 |