Influence of Combination Pharmacotherapy on Proinflammatory Gene COX-2 Expression in Skin Cancer |
| M. Woźniak, C. Paluszkiewicz, W. Kwiatek
Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342, Krakow, Poland |
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| Skin cancers are frequently occurring neoplastic changes in the human body. Among them, one of the most serious and dangerous are melanomas, meaning malignant neoplastic forms, which are characterized by a high risk of metastasis to the other organs. Currently, surgical removal is the most effective method of melanoma treatment, but due to the complicated location of tumor, such as brain or eyeballs, unfortunately not all cases undergo surgery. Second option is chemotherapy, which is very often associated with many side effects. Therefore, modern and innovative pharmacotherapy is needed. Here, we proposed a combination strategy based on administration of low doses of BRAF kinase inhibitor with mGluR1 antagonist in melanoma cell line. Detection of proinflammatory COX-2 gene expression in melanoma (SK-MEL-2) cell line was performed by the polymerase chain reaction (real-time PCR) technique. Our results showed that simultaneous administration of low doses of tested compounds significantly decreased COX-2 expression in SK-MEL-2. Conducted research may be a first step to effectively to reduce the side effects while maintaining the therapeutic effect in melanoma treatment. |
DOI:10.12693/APhysPolA.137.62 topics: melanoma, BRAF kinase inhibitor, mGluR1 antagonist, real-time PCR |