Observation of Antioxidants Activity using NMR Relaxation Measurements

D. Wierzuchowskaa, M. Witekb and B. Blicharskac aInstitute of Physics, Pedagogical University, Podchorążych 2, 30-084 Kraków, Poland bFaculty of Food Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Kraków, Poland cInstitute of Physics, Jagiellonian University, S. Łojasiewicza 11, 30-348 Kraków, Poland

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Formation of free radicals in biological solutions leads to the changes in water protons relaxation times. Therefore, NMR relaxation measurements were used to investigate kinetics of oxidation processes induced by the addition, in low amounts, of hydrogen peroxide (H2O2) to aqueous protein solution and to blood serum. The measured relaxation times were not stable over time because of the progressive formation of free radicals and their damaging action to the protein structure. The addition of antioxidants (ascorbic acid, gallic acid etc.) changed the relaxation time courses due to free radical scavenging. Similar time courses, thus anti-oxidant actions, were observed in various blood serum without antioxidant additive. Moreover, the observed kinetics of spin-lattice relaxation time (T1) depended on several factors, such as: structure and concentration of protein solutions and activity and concentration of the added antioxidants.

DOI: 10.12693/APhysPolA.133.250 PACS numbers: 76.60.-k, 76.90.+d, 76.30.Rn