Analysis of Human Lenses by Raman Microspectroscopy
C. Paluszkiewicza, P. Chaniecki b, M. Rękas c, B. Rajchel a, N. Piergies a, and W.M. Kwiatek a
aInstitute of Nuclear Physics, Polish Academy of Sciences, E. Radzikowskiego 152, 31-342 Kraków, Poland
b5th Military Hospital with Polyclinic in Kraków, Department of Ophthalmology, 30-091 Kraków, Poland
cMilitary Medical Institute, Department of Ophthalmology, 04-141 Warszawa, Poland
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A cataract is an opacity (clouding) of the normally clear lens which develops as a result of aging, metabolic disorders, trauma or heredity. The number of patients with cataract is increasing exponentially. This disease requires surgical intervention, to remove the cloudy lens and to introduce the eye lens polymer. In this work we will present analyses of degraded parts of human lens. Experimental materials were obtained from the lens removed during surgical intervention. These biological samples were measured using Almega XR Confocal Raman spectrometer (Thermo Scientific) with an excitation source of 785 nm laser line. The Raman vibrations in the spectral region of 650-1750 cm-1 were analyzed. The difference spectra revealed an excess of tryptophan, tyrosine, phenylalanine, β-sheet conformation, and molecules or molecular groups.

DOI: 10.12693/APhysPolA.129.244
PACS numbers:, 42.55.Ye, 87.64.-t