Application of HPLC-MS with Electrospray Ionization for the Qualitative and Quantitative Analysis of Antibiotics in Pharmaceutical Formulation
A. Likhtarovich a, V. Luhin a, O. Sovastei a, P. Zukowskib and M. Dado c
aBelarusian State University of Technology, 13a, Sverdlova Str., 220050 Minsk, Republic of Belarus
bLublin University of Technology, Nadbystrzycka 38a, 20-618 Lublin, Poland
cUniversity of Zilina, 1, Univerzitná Str., 01026 Žilina, Slovakia
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This study describes the application of electrospray ionization mass spectrometry in order to identify ten antibiotics (macrolides, penicillins, aminoglycosides). An optimum procedure was developed for determination of antibiotics of different grade. Positive ion spectra of most antibiotics are higher in intensity including an [M+H]+ ion and produce less fragmentation and are more informative compared to the negative ion spectra. The group of antibiotics exhibits the same characteristic fragmentation. The data base was developed for identification of antibiotics comparing of their molecular and fragment ions. The results of the study showed that the method with electrospray ionization is simple and quick which is useful in the routine determination of antibiotics and in their pharmaceutical dosage forms.

DOI: 10.12693/APhysPolA.128.901
PACS numbers: 32.10.Bi, 33.15.Ta, 82.80.-d