Studies of the Bacterial Surfaces by XPS and SEM Methods
J. Płoszaj-Pyreka,b, E. Talik b and Z. Piotrowska-Seget a
aDepartment of Biology and Environment Protection, University of Silesia, Jagiellońska 26/28, 40-032 Katowice, Poland
bAugust Chełkowski Institute of Physics, University of Silesia, Uniwersytecka 4, 40-007 Katowice, Poland
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This work presents the results of studies on bacteria Bacillus subtilis which was grown at different temperatures. The experiments were carried out using X-ray photoelectron spectroscopy and scanning electron microscope equipped with "cryo-chamber", which gives the possibility of testing biological samples in a frozen state. These methods enabled receiving images of surface topography of the bacteria and demarcated their chemical composition. After deconvolution of the lines C 1s and O 1s information concerning the intensity of the various functional groups in the bindings presented in the cell walls surface of tested strain and reallocation in relation to the temperature of incubation was obtained. These changes are visible in the form: of an additional peak in both the C 1s and O 1s in bacteria cultured in suboptimum temperature.

DOI: 10.12693/APhysPolA.125.929
PACS numbers: 87.64.-t, 71.20.Rv, 61.66.Hq, 31.50.Gh