ACTA

Application of HPLC for the Analysis of Secoisolariciresinol Diglucoside in Flaxseeds

A. Feskova^a, V. Luhin^a, V. Leontiev^a, O. Sovastei^a and T.N. Koltunowicz^b
^aBelarusian State University of Technology, Sverdlova 13a, 220050 Minsk, Republic of Belarus
^bLublin University of Technology, Nadbystrzycka 38a, 20-618 Lublin, Poland

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High-pressure liquid chromatography - electrospray ionization-mass spectrometry coupled with the PDA detector was used for the analysis of secoisolariciresinol diglucoside in flaxseeds. The research was carried out using the spectrometer "Waters" on the BDS HYPERSIL column C₁₈ 250 × 4.6 mm, with the diode-array PDA 996 and the mass-detectors Micromass ZQ 2000 with the electrospray ionization. As a mobile phase acetonitrile and water with 0.1% formic acid were used. The content of secoisolariciresinol diglucoside in the flaxseeds of 12 cultivar was determined.

DOI: 10.12693/APhysPolA.123.834
PACS numbers: 82.80.Bg, 82.80.Qx