Mechanochemical Synthesis and Characterization of II-VI Nanocrystals: Challenge for Cytotoxicity Issues

P. Baláža, R. Jardinb, E. Dutkováa, M.J. Sayaguésd, M. Baláža, G. Mojžišovác, J. Mojžišc, E. Turianicováa and M. Fabiána aInstitute of Geotechnics, Slovak Academy of Sciences, Watsonova 45, 04353 Košice, Slovakia bBruker AXS GmbH, Karlsruhe, Germany cP.J. Šafárik University, Faculty of Medicine, Košice, Slovakia dInstitute of Materials Science of Seville, Sevilla, Spain

Received: September 22, 2011; In final form: February 15, 2012

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CdSe@ZnS nanocrystals have been prepared by a two-step solid state mechanochemical synthesis. CdSe prepared from elements in the first step is mixed with ZnS synthesized from zinc acetate and sodium sulfide in the second step. The crystallite size of the new type CdSe@ZnS nanocrystals determined by X-ray diffraction Rietveld refined method was 35 nm and 10 nm for CdSe and ZnS, respectively. Energy dispersive/-transmission electron microscopy/-energy dispersive spectroscopy methods show good crystallinity of the nanoparticles and scanning electron microscopy elemental mapping illustrate consistent distribution of Cd, Se, Zn and S elements in the bulk of samples. UV-VIS spectra show an onset at 320 nm with calculated bandgap 3.85 eV. This absorption arises from the vibration modes of Zn-S bonds. The nanocrystals show the blue shift from the bandgap of bulk ZnS (3.66 eV). The synthesized CdSe@ZnS nanocrystals have been tested for dissolution, cytotoxicity and L-cysteine conjugation. The dissolution of Cd was less than 0.05 μg mL-1 (in comparison with 0.8 μg mL-1 which was evidenced for CdSe alone). The very low cytotoxic activity for selected cancer cell lines has been evidenced. CdSe@ZnS nanocrystals coated with L-cysteine are water-soluble and have a great potential in biomedical engineering as fluorescent labels.

DOI: 10.12693/APhysPolA.122.224 PACS numbers: 81.05.Dz, 81.07.Bc, 81.20.Ka, 81.20.Wk