Light Scattering Studies of Hydration and Structural Transformations of Lysozyme |
| A. Szymańska and G. Ślósarek
Department of Molecular Biophysics, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznań, Poland |
| Received: October 7, 2010; In final form: October 12, 2011 |
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| We have applied the method of dynamic light scattering to analyse the lysozyme-ethanol interaction. For low ethanol concentration (below 4.3% (v/v)) no chemical denaturation process is observed. When the ethanol concentration grows above the triggering concentration the hydrodynamic radius of lysozyme increases, indicating the structural changes within the protein molecule. The observed structural modifications are attributed to dehydration and preliminary tertiary structure modification of the protein molecule. |
| DOI: 10.12693/APhysPolA.121.694 PACS numbers: 87.15.kr, 34.50.-s |