ACTA

Characterization of Fe₃O₄ Magnetic Nanoparticles Modified with Dextran and Investigation of Their Interaction with Protein Amyloid Aggregates

M. Koneracká^a, A. Antošová^a, V. Závišová^a, G. Lancz^a, Z. Gažová^a, K. Šipošová^a, b, A. Juríková^a, K. Csach^a, J. Kováč^a, N. Tomašovičová^a, M. Fabián^c and P. Kopčanský^a
^aInstitute of Experimental Physics, SAS, Watsonova 47, 040 01 Košice, Slovakia
^bDepartment of Biochemistry, Faculty of Science, Šafárik University, Moyzesova 11, 040 01 Košice, Slovakia
^cInstitute of Geotechnics, SAS, Watsonova 45, 040 01 Košice, Slovakia

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Functionalised magnetic nanoparticles composed of Fe₃O₄ particles stabilised by sodium oleate and subsequently modified with dextran (MFDEX) were prepared by the co-precipitation method. Their morphology and particle size distribution were observed by scanning electron microscopy and photon cross correlation spectroscopy. In order to confirm the modification of magnetite surface with dextran physical techniques, including infrared spectroscopy, thermal analysis, and magnetic measurement, were used. Finally, the effect of MFDEX on amyloid fibrillar aggregates of human insulin and hen egg white lysozyme, typical amyloidogenic proteins, was investigated. In vitro interaction of MFDEX with protein amyloid fibrils resulted into destruction of amyloid aggregates. The anti-amyloid activity makes MFDEX of potential interest as therapeutic agent against amyloid-related diseases.

DOI: 10.12693/APhysPolA.118.983
PACS numbers: 75.50.Mm, 87.14.em, 87.19.xr