Fourier Transform Near Infrared Raman Spectroscopy in Studies on Connective Tissue

M. Połomskaa, L. Kubiszb, R. Kalawskic, G. Oszkinisd, R. Filipiakc and A. Mazurekc aInstitute of Molecular Physics, Polish Academy of Sciences, M. Smoluchowskiego 17, 60 179 Poznań, Poland bDepartment of Biophysics, Poznań University of Medical Sciences, Fredry 10, 61-701 Poznań, Poland cDepartment of Cardiac Surgery, Municipal Hospital, Szkolna 8/12, 61-417 Poznań, Poland dDepartment of General and Vascular Surgery, Poznań University of Medical Sciences, Poznań, Poland

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Studies of tissue such as bones and veins often are carried out by means of microscopic and ultrasonographic methods. Therefore studies of changes caused by pathological or physical factors often are limited to morphological level. Application of Raman spectroscopy permits to associate morphological changes and molecular changes. The paper presents preliminary studies of temperature and ionizing radiation effects on Raman spectra of bone and collagen. Furthermore, effects of pathological factors on Raman spectra of human veins had been studied. It was noticed that heating of bone collagen up to 110°C gives the reversible changes in spectra. In the case of γ irradiation, any effects were observed up to the dose of 1 MGy on mineral component of bone, whereas the influence on collagen was observed for doses higher than 100 kGy. The Raman spectra of varicose vs. normal veins permit to conclude some important changes observed for veins from chronic venous insufficiency patients.

DOI: 10.12693/APhysPolA.118.136 PACS numbers: 78.30.-j, 78.30.Jw, 61.80.Ed, 61.82Pv, 81.05.-t, 87.-g, 87.93.-j, 87.64.K-