Application of μ-FTIR-SR Spectroscopy to Prostate Tissue Analysis |
| W.M. Kwiatek a, C. Paluszkiewicz b, A. Banaś a, A. Kisiel c, M. Podgórczyk a, A. Marcelli d, M. Cestelli Guidi d,e and M. Piccinini d,e
a Institute of Nuclear Physics, PAS, Radzikowskiego 152, 31-342 Kraków, Poland b AGH University of Science and Technology, al. Mickiewicza 30, 30-059 Kraków, Poland c Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Kraków, Poland d INFN - Laboratori Nazionali di Frascati, Via E. Fermi 40, I-00044 Frascati (Rome), Italy e Universita' Roma Tre, Dip. Scienze Geologiche, L. go S. Leonardo Murialdo 1, 00146 Rome, Italy |
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| The infrared spectromicroscopy is a quite recent technique rapidly developing thanks to the availability of new instruments and new brilliant synchrotron radiation sources in different areas and in particular to biomedical researches. In order to achieve a diffraction limited spatial resolution in tissue samples, we performed experiments at SINBAD, the synchrotron infrared beamline of the Laboratori Nazionali di Frascati. We characterized the chemical composition of prostate tissue samples taken from patients affected by prostate cancer disease. Different sizes of the pinholes were considered for the measurements. In the case of prostate tissue sections the results show the possibility to determine the intensity ratio of the CH2 and CH3 bands set at 2930 cm-1 and 2960 cm-1, respectively. Experiments were also performed with a pinhole of 5 μm of diameter and the differences in both histological and chemical compositions of such samples were determined. |
| DOI: 10.12693/APhysPolA.115.602 PACS numbers: 87.64.km, 87.64.kp, 87.64.Ee, 87.85.jf |