Sulphur XANES Analysis of Cultured Human Prostate Cancer Cells

W.M. Kwiatek a, M. Podgórczyk a, Cz. Paluszkiewicz b, A. Balerna c and A. Kisiel d a The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342 Cracow Poland b AGH University of Science and Technology, al. Mickiewicza 30, 30-059 Cracow, Poland c INFN - Laboratori Nazionali di Frascati, Via E. Fermi 40, I-00044 Frascati, Italy d Institute of Physics, Jagiellonian University, Reymonta 4, 30-059 Cracow, Poland

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Received: 24 09 2007;

Prostate cancer is one of the most commonly diagnosed cancers in men throughout the world. It is believed that changes to the structure of protein binding sites, altering its metabolism, may play an important role in carcinogenesis. Sulphur, often present in binding sites, can influence such changes through its chemical speciation. Hence there is a need for precise investigation of coordination environment of sulphur. X-ray absorption near edge structure spectroscopy offers such possibility. Cell culture samples offer histologically well defined areas of good homogeneity, suitable for successful and reliable X-ray absorption near edge structure analysis. This paper presents sulphur speciation data collected from three different human prostate cancer cell lines (PC-3, LNCaP and DU-145). Sulphur X-ray absorption near edge structure analysis was performed on K-edge structure. The spectra of cells were compared with those of cancerous tissue and with organic substances as well as inorganic compounds.

DOI: 10.12693/APhysPolA.114.463 PACS numbers: 87.64.kd, 87.19.xj