Enhancement of FITC Fluorescence by Silver Colloids and Silver Island Films |
| S. Kruszewski, T. Wybranowski, M. Cyrankiewicz, B. Ziomkowska and A. Pawlaczyk
Medical Physics Division, Biophysics Department, Collegium Medicum of Nicolaus Copernicus University, Jagiellońska 13, 85-067 Bydgoszcz, Poland |
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| Received: 27 03 2007; in final form: 15 02 2008; |
| Silver colloids and silver island films as fluorescence enhancement systems are the subject of this paper. Enhancement of fluorescence of molecules placed near metallic nanoparticles is a result of resonance interaction of exciting and emitted lights with surface plasmons. Two models explaining this phenomenon are presented. Processes of silver colloids and silver island films forming are described. Results of extinction, absorption and scattering studies of fluorescence enhancement systems are presented. The influence of these systems, i.e. silver colloids and silver island films on the fluorescence of fluorescein-5-isothiocyanate was studied. 7-fold increase in fluorescence intensity of fluorescein-5-isothiocyanate molecules placed near silver nanoparticles was observed. |
| DOI: 10.12693/APhysPolA.113.1599 PACS numbers: 33.20.Fb, 33.20.Kf, 33.50.-j |