Irradiation of Living Cells with Single Ions at the Ion Microprobe SNAKE

A. Hauptnera, T. Cremerb, M. Deutschc, S. Dietzelb, G.A. Drexlerc, C. Greubela, V. Habled, R. Krückena, R. Löwec, H. Strickfadenb, G. Dollingerd and A.A. Friedlc aPhysik Department E12, Technische Universität, München, Germany bDepartment Biologie II, Ludwig-Maximilians-Universität, München, Germany cStrahlenbiologisches Institut, Ludwig-Maximilians- Universität, München, Germany dInstitut für Angewandte Physik und Messtechnik (LRT 2), Universität der Bundeswehr, München, Germany

Full Text PDF

Received: 2005 20 05;

The irradiation setup at the ion microprobe SNAKE is used to irradiate living cells with single energetic ions. The irradiation accuracy of 0.55μm and respectively 0.40μm allows to irradiate substructures of the cell nucleus. By the choice of ion atomic number and energy the irradiation can be performed with a damage density adjustable over more than three orders of magnitude. Immunofluorescence detection techniques show the distribution of proteins involved in the repair of DNA double-strand breaks. In one of the first experiments the kinetics of appearance of irradiation-induced foci in living HeLa cells was examined. In other experiments a new effect was detected which concerned the interaction between irradiation events performed at different time points within the same cell nucleus.

DOI: 10.12693/APhysPolA.109.273 PACS numbers: 29.27.Eg, 61.80.Jh, 87.16.Sr, 87.50.Gi