Paramagnetic Centers in DOPA-Melanin-Dihydrostreptomycin Complexes

E. Buszmana, B. Pilawab, M. Zdybelb, D. Wrześnioka, A. Grzegorczyka and T. Wilczokc aDepartment of Pharmaceutical Chemistry, School of Pharmacy, Medical University of Silesia Jagiellońska 4, 41-200 Sosnowiec, Poland bDepartment of Medical Physics, School of Pharmacy, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland cDepartment of Molecular Biology and Medical Genetics, School of Pharmacy, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland

Full Text PDF

Received: 24 04 2005;

DOPA-melanin-dihydrostreptomycin complexes with drug concentrations 1×10-4-1×10-2 M were examined by the use of electron paramagnetic resonance spectroscopy at X-band (9.3 GHz). Dihydrostreptomycin was chosen for studies, because this aminoglycoside antibiotic causes strong toxic effects in organism. It was stated that dihydrostreptomycin generates o-semiquinone free radicals with g=2.0038 in melanin. Free radicals formation increases with increase in the antibiotic concentration. Changes of EPR lines with microwave powers pointed out that slow spin-lattice relaxation processes exist in DOPA-melanin and in its complexes with dihydrostreptomycin. The measured EPR lines were homogeneously broadened.

DOI: 10.12693/APhysPolA.108.353 PACS numbers:61.66.Hq, 76.30.Rn